1992 Volume 1992 Issue Supplement59 Pages 30-34
The human maxillary cancer cell line, MMSI-1, was established from the xenograft which was implanted in nude mice and had been maintained for more than five years. Its characteristics were examined, MMSI-1 showed stable growth and the population doubling time was 1.9 days. Intravenous injection of MMSI-1 tumor cells produced lung metastases in nude mice. Southern blot hybridization showed seven-fold c-myc amplification; there was no overexpression of N-myc, erb B and erb B2. The lysate of MMSI-1 cells shortened the plasma recalcification time, but no thromboplastic activity was found in this lysate.
There has been no previous report on human head and neck cell lines w h ich can metastasize to the lung by intravenous inoculation of cancer cells. Therefore, the most conspicuous property of MMSI-1 seemed to be its metastasizing ability.
