Immunohistochemical Technique on Celloidin-Embedded Human Temporal Bone Sections

Abstract

A new technique which allows immunohistochemical detection of proteins in routinely processed, colloidin-embedded human temporal bone sections was recently developed by Shi, et al. This technique enabled us to immunohistochemically study numerous temporal bone sections preserved for a long period. We examined the reliability and usefulness of this technique using three monoclonal antibodies to cytokeratin, vimentin and neuron specific enolase (NSE). These antibodies showed definite positive staining at the inner ear as previously described by many reaerchers. This technique may prove very useful in the immunohistochemical study of the temporal bone in Heidenhain-SuSa-fixed, acid- decalcified, celloidin-embedded sections.