Abstract
The infectivity of Bombyx mori cypovirus 1 (BmCPV-1) in four different lepidopteran cell lines (BmN4, SES-Bm-130, TUAT-Spli-221 and IPLB-Sf-21) was analyzed. The B. mori derived BmN cell line showed the highest susceptibility to a BmCPV-1 inoculum that was prepared by dissolving polyhedra in alkaline solution and removing polyhedrin after neutralization with HCl. Incubation of the BmN cells at 30°C in TC-100 medium containing 10% FBS was optimal for the formation of BmCPV-1 polyhedra. The formation of polyhedra was virus-dose dependent. In BmN cells, polyhedra were detected at 3 days after inoculation at 25°C and the number of cells containing polyhedra increased up to 8 days post infection. There remained many apparently healthy cells in the BmN culture even at 13 days after inoculation, indicating that the transmission of BmCPV-1 from infected to healthy cells was rare or did not occur. Adsorption time of the virus to the cell was estimated to be more than 16 hours suggesting that low affinity between the virus and cell was one reason for low infectivity of BmCPV-1 to cultured cells. The development of an infection system for BmCPV-1 in cultured cells is also discussed.
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