Abstract
To investigate the effect of a promoter sequence for detecting enhancer activity in the silkworm Bombyx mori, we constructed six different GAL4 vectors under the control of different minimum promoters. In addition, a homozygous UASGFP strain was constructed and used to test expression of the GAL4 gene. Green fluorescent protein (GFP) expression was compared in the transgenic strains with the different GAL4 gene inserts. The most prominent difference in the expression pattern in the different strains was observed when the Bombyx cytoplasmic actin gene (BmA3) promoter was used. We concluded that using the GAL4 gene with the BmA3 promoter would be the most useful way for detecting the positional effect of genes inserted in the silkworm.
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