Mapping Analysis of Carotenoid-binding Protein of Bombyx mori by Restriction Fragment Length Polymorphism

Wajiro Hara; Stephen Sosnicki; Yutaka Banno; Hirofumi Fujimoto; Naoko Takada; Hideaki Maekawa; Hiroshi Fujii; Michael A. Wells; Kozo Tsuchida

doi:10.11416/jibs.76.3_149

Regular Articles

Mapping Analysis of Carotenoid-binding Protein of Bombyx mori by Restriction Fragment Length Polymorphism

Wajiro Hara, Stephen Sosnicki, Yutaka Banno, Hirofumi Fujimoto, Naoko Takada, Hideaki Maekawa, Hiroshi Fujii, Michael A. Wells, Kozo Tsuchida

Author information

Wajiro Hara
National Institute of Agrobiological Sciences
Stephen Sosnicki
Department of Biochemistry and Molecular Biophysics, University of Arizona
Yutaka Banno
Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
Hirofumi Fujimoto
Division of Radiological Protection, National Institute of Infectious Diseases
Naoko Takada
Division of Radiological Protection, National Institute of Infectious Diseases
Hideaki Maekawa
Division of Radiological Protection, National Institute of Infectious Diseases
Hiroshi Fujii
Institute of Genetic Resources, Faculty of Agriculture, Kyushu University
Michael A. Wells
Department of Biochemistry and Molecular Biophysics, University of Arizona
Kozo Tsuchida
Division of Radiological Protection, National Institute of Infectious Diseases

Keywords: CBP, Yellow hemolymph, Bombyx mori, RFLP, mapping, Y-gene

JOURNAL FREE ACCESS

2007 Volume 76 Issue 3 Pages 3_149-3_154

DOI https://doi.org/10.11416/jibs.76.3_149

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Abstract

The gene for carotenoid binding protein (CBP) in the silkworm, Bombyx mori, was mapped. The linkage group for the CBP gene was identified by scanning linkage analysis of the restriction fragment length polymorphism (RFLP) pattern, and its genetic mapping with respect to known molecular markers within the group was established. The CBP gene was found to be linked to RFLP linkage group 19 which is equivalent to the phenotypic linkage group 2. The linkage map at 55cM in length was constructed for this linkage group, with CBP mapped at 21cM in length. When compared to a phenotypic map of chromosome 2, this result suggests that the gene that encodes CBP is the Y- gene.

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