Application of Real Time PCR for the Quantitative Detection of Radiation-induced Genomic DNA Strand Breaks

Emiko Yamauchi; Ritsuko Watanabe; Miyoko Oikawa; Hirofumi Fujimoto; Akinori Yamada; Kimiaki Saito; Masahiro Murakami; Kazuo Hashido; Kozo Tsuchida; Naoko Takada; Hajime Fugo; Zhenli Tu; Hideaki Maekawa

doi:10.11416/jibs.77.1_17

Regular Articles

Application of Real Time PCR for the Quantitative Detection of Radiation-induced Genomic DNA Strand Breaks

Emiko Yamauchi, Ritsuko Watanabe, Miyoko Oikawa, Hirofumi Fujimoto, Akinori Yamada, Kimiaki Saito, Masahiro Murakami, Kazuo Hashido, Kozo Tsuchida, Naoko Takada, Hajime Fugo, Zhenli Tu, Hideaki Maekawa

Author information

Emiko Yamauchi
Division of Radiological Protection and Biology, National Institute of Infectious Diseases
Department of Biological Production, Tokyo University of Agriculture and Technology
Ritsuko Watanabe
Radiation Effect Analysis Group, Japan Atomic Energy Agency
Miyoko Oikawa
Division of Radiological Protection and Biology, National Institute of Infectious Diseases
Division of Science and Technology, Shinshu University
Hirofumi Fujimoto
Division of Radiological Protection and Biology, National Institute of Infectious Diseases
Akinori Yamada
Center of Molecular Biosciences, University of Ryukyus
Kimiaki Saito
Radiation Effect Analysis Group, Japan Atomic Energy Agency
Masahiro Murakami
Radiation Hazard Research Group, National Institute of Radiological Sciences
Kazuo Hashido
Division of Radiological Protection and Biology, National Institute of Infectious Diseases
Kozo Tsuchida
Division of Radiological Protection and Biology, National Institute of Infectious Diseases
Naoko Takada
Division of Radiological Protection and Biology, National Institute of Infectious Diseases
Hajime Fugo
Department of Biological Production, Tokyo University of Agriculture and Technology
Zhenli Tu
College of Animal Sciences, Zhejiang University
Hideaki Maekawa
Division of Radiological Protection and Biology, National Institute of Infectious Diseases

Keywords: single strand break, radiation, scavenger, real-time PCR, plasmid DNA, Monte Carlo simulation, Bombyx mori

JOURNAL FREE ACCESS

2008 Volume 77 Issue 1 Pages 1_17-1_24

DOI https://doi.org/10.11416/jibs.77.1_17

Details

Abstract

The frequency of single strand breaks (SSBs) occurring on both strands of the pBR322 plasmid DNA region flanked by a pair of primers used for polymerase chain reaction (PCR) amplifications was determined after irradiation with ¹³⁷Cs γ rays. We verified that real time PCR is suitable for the detection and quantitative analysis of SSBs caused by γ ray irradiation. The utility of this approach was also supported by the comparison of the practical experimental data with the Monte Carlo simulation. The potential application of this PCR method for the detection of genomic DNA damage was also confirmed.

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