Abstract
A clonal analysis system in the silkworm, Bombyx mori, was developed to achieve expression of genes of interest in portions of tissues. We established two types of transgenic silkworm strains: one expresses yeast FLP recombinase under the control of a heat shock promoter, and the other possesses the DsRed gene interrupted with the GFP gene flanked at both ends with two FLP recombinase target (FRT) sites. The F1 eggs between the two strains were heat-shocked to induce FLP recombinase, which catalyzed intrachromosomal recombination to excise the GFP gene, resulting in the expression of DsRed in randomly formed cell clones.
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