2019 Volume 88 Issue 2 Pages 2_027-2_030
In this paper, we report on the specific spore germination conditions of the microsporidia Trachipleistophora haruka (Old name Trachipleistophora sp. OSL-2012-10 was reported by Shigano et al. (2015)) isolated from the common cutworm, Spodoptera litura from the Ogasawara Islands, Japan. We analyzed germination rates in varying salt conditions and found that alkaline stress was required for spore germination. Nosema bombycis NIS-001 of the experiment control germinated regardless of temperature only by treatment of 0.1 M KOH or 0.1 M KCl+3% (w/v) H2O2. However, Trachipleistophora haruka treatment by 0.85% NaCl solution at 25°C showed germination behavior similar to treatment of 0.1 M KOH and 0.1 M KCl+3% (w/v) H2O2. The results indicated that this microsporidia requires sodium or potassium ions to germinate.
