Journal of the Japan Institute of Energy
Online ISSN : 1882-6121
Print ISSN : 0916-8753
ISSN-L : 0916-8753
Original Paper
Adsorption of Trichoderma reesei Cellulase on Softwood Lignin-based Lignophenol
Hirosh NONAKAHiroaki TANAKAMasamitsu FUNAOKA
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2011 Volume 90 Issue 10 Pages 977-981

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Abstract

Lignophenol is a lignin-based polymer which highly conserves the native lignin structure. Through simple adsorption experiments between Trichoderma reesei cellulase and softwood lignophenol synthesized from Hinoki cypress and p-cresol, several ideas were obtained on non-productive cellulase binding to lignin expected during enzymatic saccharification of cellulosic substrates. Cellulase is promptly bound to lignin within 10 min. The adsorption reaches a saturated value, indicating that cellulase adsorption on lignin is not multi-layered. Maximum adsorption is observed at around the isoelectric point of cellulase and, at the same time, phenolic hydroxyl groups in lignin seem to enhance cellulase binding. Cellulase bound to lignin maintains its enzymatic activity and probably functions as an immobilized cellulase in saccharification liquor.

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© 2011 The Japan Institute of Energy
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