2012 Volume 91 Issue 9 Pages 915-922
Effects of delignification treatment on the enzymatic saccharification of grass biomass (rice straw and sugarcane bagasse) were investigated. The delignification treatment improved the efficiency of the enzymatic saccharification. The yields of glucose and xylose from the delignified rice straw by enzymatic saccharification at low cellulase loading (2 FPU/g-substrate) were 75 and 65 %, respectively. Furthermore, combining delignification and hot compressed water treatment at 140°C, the yields of glucose and xylose was increased to 95 and 80 %, respectively. On the other hand, the yields of glucose and xylose from the sugarcane bagasse combining delignification and hot compressed water treatment by enzymatic saccharification were 55 and 70 %, respectively. It is shown that the high cellulase loading (10 FPU/g-substrate) was needed for obtaining high monosugar yield from sugarcane bagasse.