2016 Volume 95 Issue 10 Pages 930-936
Enzymatic hydrolysis by cellulase has potential as an environment-friendly technology for producing bioethanol from lignocelluloses, but its production cost remains expensive. Therefore, several studies have examined methods in reducing the cost of enzymatic hydrolysis, and one of the most effective ways is to reuse enzymes. In this study, we developed a method for evaluating the cellulase activity in enzymatic hydrolysis residues for efficient enzyme reuse. Approximately 70% the of cellulase activity remained in the solid residue after 70% of the glucan was hydrolyzed, and 22% of cellulase activity still remained in the solid residue after 99% of glucan was hydrolyzed. Other experiments were performed in order to examine the application of our proposed method for practical reuse of enzymes. The results indicate that enzymes were recovered from the residues as we estimated by our method. Thus our method is efficient for evaluating cellulase activity in enzymatic hydrolysis residues.