Abstract
Ethanol production from lignocellulosic biomass has attracted attention for utilization as an alternative fuel for internal combustion. Pretreatment is the first step in the conversion of lignocellulosic biomass to ethanol. Fermentation inhibitors generated by hydrothermal pre-treatment have an inhibitory influence on yeast growth and fermentation. Simultaneous saccharification and fermentation (SSF) is a promising, cost-effective method demonstrated in this study. We showed that yeast acquired thermotolerance in preculture at 35 °C and the thermotolerant yeast developed tolerance to 5-HMF by adding 5-HMF in preculture. Thus, we showed that Saccharomyces cerevisiae has the ability to tolerate stress caused by multiple factors including heat and inhibitors.
