Hydrogen Production from Sho-chu Post-Distrillation Slurry by Newly Isolated Strain, Clostridium sp.JPCC H-3

Abstract

The barley-, sweet potato-, rice sho-chu post-distillation slurry are organic acid rich waste which annually produced from sho-chu manufactures. The biological hydrogen production from sweet potato sho-chupost-distillation slurry by newly isolated bacteria was demonstrated. The bacteria that can produce hydrogen were isolated from 187 location sites. The anaerobic bacterium, Clostridium sp.JPCC H-3 was found the highest hydrogen producer from Ametaki. The maximum hydrogen production rate of Clostridium sp.JPCC H-3 was obtained at 5.35±0.19ml/5ml-solution for 24hr under 37°C and dark conditions. It was 1.4 times higher than that of Clostridium butyricum NRBC3315 as comparative strain. Moreover, organic acids such as acetic acid and lactic acid were substrates for hydrogen production by Clostridium sp.JPCC H-3. The sweet potato sho-chu post-distillation slurry could be use substrates for hydrogen production and Clostridium sp.JPCC H-3 will be one of the suitable candidates for hydrogen production from sweet potato sho-chu postdistillation slurry.