Abstract
Three bio-ethanol production processes from lingo-cellulose, saccharification using concentrated sulfuric acid, using diluted sulfuric acid and enzyme, and combination of milling pretreatment and enzymatic saccharification, were selected, and designed to estimate material and energy balance. The process of combination of milling pretreatment and enzymatic saccharification shows the potential of the highest ethanol yield. The process of saccharification using concentrated sulfuric acid requires the lowest process energy. For the process of combination of milling pretreatment and enzymatic saccharification, the milling using disk-mill decreases drastically the milling energy rather than using ball-mill, and the required process energy is even as the other processes. The rough ethanol production costs are discussed.