O-105 Improvement of intermolecular interaction between cellulase and cellulose for the efficient enzymatic saccharification of cellulosic biomass

Abstract

Hydrolysis of crystalline celluloses with different crystalline polymorphs, cellulose I and III_I, was monitored using fungal cellobiohydrolases. When cellobiose production by cellobiohydrolases was compared between cellulose I and III_I, apparent hydrolytic advantage was observed in hydrolysis of cellulose III_I than that of cellulose I. The amount of high-affinity bound Trichoderma reesei Cel7A was increased on cellulose III_I, suggesting that strong interaction between cellulase and cellulose improves efficiency of saccharification.