Abstract
The purpose of this study is the development of environmentally-friendly pretreatment for ethanol production from lignocellulose. Hot compressed water-treatments (HCWT) in two kinds of the mixed solvent (water (W)/ethanol (EtOH)/acetic acid (A), and W/ethylene glycol (EG)/A) were investigated, respectively, for the enzymatic hydrolysis of Japanese cypress in this study. It was found that ball milling need in the HCWT using W/EtOH/A. The suitable conditions of the pretreatments were as follows; at 140℃ in W/EtOH/A (25/75/1 in-feed weight ratio); at 170℃ in W/EG/A (25/75/1 in-feed weight ratio), respectively.