Abstract
Larvae of the cryptomeria bark borer, Semanotus japonicus (Lacordaire) (Coleoptera: Cerambycidae), were aseptically reared on an artificial diet. A single larva, hatched from a surface-sterilized egg, was placed in each flask containing diet. During the larval period, it was not necessary to transfer the larva to a new flask. No microorganisms were observed growing in any of the flasks. Ultimately, 50% of the larvae emerged, which is a rate similar to that obtained with non-aseptic rearing on an artificial diet. Moreover, all of the adult females oviposited eggs and approximately 80% of these eggs hatched. These results indicate that successive generations of the borer can be reared in this manner.