Abstract
Electrophoretic patterns of hemolymph proteins in the larvae of fall webworm, Hyphantria cunea, which had been reared from field-collected eggs on the leaves of different food plants, were studied. By means of agarose-gel electrophoresis, the hemolymph protein was resolved into three main fractions, A, B, and C. Although, in the hemolymph of larvae reared on leaves of Morus bombycis, Salix subopposita, Juglans mandshurica, and Acer negundo, the three main fractions of protein were clearly detected, B fraction was undetectable in the larvae reared on the other 8 plants, namely Cornus controversa, Sambucus sieboldiana, Diospyros kaki, Prunus yedoensis, Populus nigra, Rubus palmatus, Liquidambar formosana, and Styrax japonica. Thus, the B-protein fraction would appear in the electropherogram of hemolymph from larvae supposed to be reared with suitable food plants for the larval growth. When the food plants, such as S. sieboldiana and D. kaki, were replaced to feed the larvae with M. bombycis, B-protein fraction in the larval hemolymph became detectable about three days thereafter.
