Abstract
1. Coenzyme A and choline acteylase are essential for the synthesis of acetylcholine in the eggs of the rice stem borer, Chilo suppressalis, and of the cabbage armywarm, Barathra brassicae.
2. The absence of synthetic activity of acetylcholine in these eggs of early stage is due to the lack of the terminal enzyme, choline acetylase, nevertheless coenzyme A is present during the course of their embryonic development.
3. The fluctuations of acetylcholine and the related factors in the eggs of insects are shown in diagramatically.