Abstract
Sedimentation tests were performed by adding RFP purified from the silkworm digestive juice to NPV extracted from the nuclear-polyhedral body of a diseased silkworm by two methods, a capillary tube and an agglutination plate. The white line of the precipitate clearly occurred at the border of the RFP and NPV layers in buffer solutions when they were carefully introduced into the capillary tube. The precipitate was also observed in the pH range of 7.5-8.5 when these two solutions were mixed in the bottom of the agglutination plate. RFP also reacted with silkworm flacherie virus (FV) to precipitation, but the reactions with tabacco mosaic virus (TMV) and Autographa california NPV (AcNPV) were negative. RFP did not precipitate with proteins such as serum albumine, histone, and the protein of the polyhedral body of silkworm NPV.
