Properties of Somatic Soluble Antigens of Beauveria bassiana

Abstract

Immunological comparison among 3 insect non-pathogenic fungi (Fusarium oxysporum, F. laleritium, Diaporthe nomurai), 4 insect pathogenic fungi (Beauveria brongniartii, Paecilomyces fumosoroseus, P. farinosus, Nomuraea rileyi) and 3 serotypes of blastospores in B. bassiana was made using immunodiffusion tests. In agar-gel diffusion tests, somatic soluble antigens obtained from F. oxysporum, F. lateritium, and D. nomurai did not react with anti-B. bassiana F1 (serotype B₁) serum. Somatic soluble antigens obtained from B. brongniartii, P. fumosoroseus, and P. farinosus gave 1-3 precipitin lines against anti-B. bassiana F1 (serotype B₁) serum whereas the antigen obtained from N. rileyi did not react with anti-B. bassiana F1 (serotype B₁) serum. In agar-gel diffusion tests using somatic soluble antigens of the 3 serotypes in B. bassiana, one or two specific antigens for B. bassiana F1 (serotype B₁) and common antigens for the 3 serotypes were detected. The results of immunodiffusion tests with somatic soluble antigens were very similar to those with the serotypes of blastospores in B. bassiana.