Abstract
Virus-like particles were isolated from the Brown-Winged Green Bug, Plautia stali. Diseased nymphs revealed typical symptoms of virus infection. Re-infection of healthy nymphs with haemolymph of diseased nymphs resulted in typical disease symptoms. Infected nymphs died within several days with severe symptoms. The haemolymph maintained virulence for nymphs after filtration of 0.22-μm pore size and after serial passage through nymphs. One opalescent band was seen after sucrose density-gradient centrifugation. The band had virulence to nymphs and contained spherical particles estimated about 30nm in diameter by electron microscopy. These results indicated that the particles are a virus.
