2024 Volume 30 Issue 1-2 Pages 19-26
Purpose: Oral dryness caused by side effect of medicines has been increasing in elderly. The aim of this study was to investigate the relationship between protein ingredient and salivary volume on oral dryness patients caused by calcium blocker and healthy elderly.
Methods: Six patients taking calcium blocker and six healthy elderlies were enrolled. Unstimulated salivary volume and protein concentration were investigated. iTRAQ proteomic analysis was performed to detect all salivary proteins. The relation between salivary volume and the ratio of the intensities of western blotting product to that of β-Actin was investigated. The relation between protein concentration per salivary volume and the ratio of the intensities of western blotting product to that of β-Actin was also examined.
Results: As the result of iTRAQ proteomic analysis, 968 kinds of salivary proteins were detected. Calmodulin-like protein 3 was included with a constant rate in the saliva of oral dryness patients, and less likely to detect on the saliva of healthy elderly. Glutathione S-transferase P was detected with a constant rate in the saliva of oral dryness patients, but it also included in saliva of healthy elderly.
Conclusions: These results indicated that calmodulin-like protein 3 tended to be detected on the saliva of oral dryness patients caused by calcium blocker.
