Japanese Journal of Biometrics
Online ISSN : 2185-6494
Print ISSN : 0918-4430
ISSN-L : 0918-4430
Original Article
Mapping of QTL in Recombinant Inbred Lines Derived from an F2 by Repeated Cycles of Self-fertilization
Takeshi Hayashi
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1999 Volume 20 Issue 1 Pages 57-75

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Abstract
On the basis of a high-density genetic map with a large number of molecular markers quantitative trait loci (QTLs) can be finely mapped. However, the information of a dense map cannot be fully utilized by early segregating generations such as an F2 and a backcross due to insufficient number of recombination events among closely linked markers. One needs to advance generations to increase the number of recombination events in order to take advantage of a dense map. In this paper the utility of populations in advanced generations such as F5, F6 or recombinant inbred lines derived from F2 by repeated cycles of self-fertilization in mapping of QTLs is discussed. The effectiveness of mapping a QTL based on advanced generations is evaluated in a simple regression model. The power of detection of a QTL is enhanced and the lengths of support intervals of map location of a QTL are reduced as generations advances, especially the efficiency of detecting and locating a QTL is significantly improved in F3-F5 in comparison with F2. Moreover a multiple regression model based on a large number of markers is devised to separate two linked QTLs. The ability of dissolving multiple linked QTLs is increased as generations advance.
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© 1999 The Biometric Society of Japan
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