Abstract
The interaction of eugenol (EUG) and its related compound (guaiacol, phenol and safrole) with dipalmitoylphosphatidylcholine (DPPC) was investigated by differential scanning calorimetry (DSC) and by NMR spectroscopy, to elucidate their activity at the membrane level. EUG with phenolic OH and allyl group markedly lowered the endothermic phase transition temperature (Tm) compared to that of guaiacol, phenol and safrole.
1H-NMR spectra of DPPC/EUG (2:1 molar ratio) and DPPC/cholesterol/EUG (2:1:1) showed EUG signals markedly broadened in a wide temperature range (18〜52℃). EUG signals at 18℃ shifted significantly to a higher field (shielding). It was concluded that EUG has a strong interaction with DPPC liposomes.
