Observation of Structure of Human Teeth during Immersing in Liquid by Atomic Force Microscopy

Abstract

Untreated enamel and dentin were observed in air, water and acid agents consecutively by atomic force microscopy(AFM).AMF allowed observation in situ of the change in morphology during the etching process.The change before and after etching could be observed for the same spot in the same specimen using the newly designed syringe for exchanging liquids.In 10% citric acid the smear layers of both enamel and dentin disappeared wihin 30 seconds.Then, enamel prisms or dentinal tubles appeared and demineralization progressed with time.