Abstract
The in vitro early cellular responses of osteoblast-like cells(MC3T3-E1)were studied on titanium surfaces with different micromorphologies.Surfaces with a regular mophology were produced by polishing with 1500-grit, 400-grit or 60-grit emery papers, and those with an irregular morphology were produced by wire type electric discharge maching(Wire-EDM).The surface roughness(Rmax)of the surface with 1, 500-grit, 400-grit, 60-grit, and Wire-EDM was 0.8, 2.6, 8.0 and 20.9μm respectively.Early cellular attachment for the remaining time period of 60 minutes was calculated by measurement of the lactic dehydrogenase(LDH)activities of the attached cells.Significantly higher levels of cellular attachment were found on the irregular surface with Wire-EDM than the regular surface with emery polishing.Among the surfaces with emery polishing, surfaces with a higher surface roughness had a tendency to have higher attachment.Attached cells were observed to already extend flopodia to form a bridge-like anchor between indentations of the irregular surfaces for 60 minutes on the surface with Wire-EDM.These findings suggested that the surface micromorphologies influenced the cellular responses of the osteoblast-like cells and further studies on longer time cellular responses are required.
