Abstract
The cytotoxicity of commercial denture lining materials to a tissue model (MATREX) which is an in vitro reconstructed cell culture system, was tested and evaluated by the lactate dehydrogenase and MTT assay. Eight cold-curing acrylic materials yielded strong cytotoxicity to the tissue model. However, the heat-curing type was hardly cytotoxic. A silicone material showed moderate cytotoxicity and a fluorine-containing material slight cytotoxicity. On the other hand, three light-curing type materials showed moderate to strong cytotoxicity prior to light irradiation, which decreased cytotoxicity. The results of cell recovery after exposure of extracts of the cold-curing acrylic materials remained low to moderate. The heat-curing acrylic material did not affect the cell recovery. The silicone material showed moderate cell recovery and the fluorine-containing material a slightly higher rate of cell recovery. The light-curing type materials had a similar effect on cells in the tissue model. Cell recovery was similar in three repeated extractions of materials. Some suggestions might be given in in vitro testing of materials in close contact with the oral mucous membrane, as are denture lining materials.
