Abstract
Down's syndrome is a chromosome abnormality that occurs with high frequency. Recently, the average life expectancy of these patients is increasing because of advances in nursing care for handicapped people. Therefore, the necessity to consider the cytotoxicity levels of dental materials for the Down's syndrome patient will increase. It is possible that the toxicity of various dental materials to Down's syndrome cells differs from that to normal human cells, but there is no basis for determining the appropriate materials for these patients. Therefore, the cytotoxicity levels of root canal filling materials, pulp capping agents, and root canal remedial agents were compared between fibroblast-like Down's syndrome cells (Detroit 539) and normal human cells (HUG-F) - Cell viability was measured by the standard monolayer culture and three-dimensional culture with a Type 1 collagen gel matrix. As a result, the Detroit 539 cell line showed a weak cytotoxic response to most materials compared with that shown by the HUG-F cell line, and the difference in the cell proliferation degree for both cells was considered. Both IC50 (50% inhibiting concentration) of the standard monolayer culture method and the cell viability on three dimensional culture showed a remarkable correlation between the two cell lines. Therefore, the utility of cytotoxicity testing using the Detroit 539 cell line is suggested.
