Abstract
The correlation of between cytotoxicity testing using the neutral red method and a new method for toxicity test using changes in membrane fluidity in PL cells derived from human periodontal membrane was examined. The ranking order of cell toxicity when cells were treated with acrylic resin monomers for 3hrs was trimethylolpropane trimethacrylate (TMPT)≥epoxy methacrylate (D-GMA)>1,4-buthane diol dimethacrylate (BD)>1,3-buthylene glycol dimethacrylate (BG)>ethylene glycol dimethacrylate (1G)>triethylene glycol dimethacrylate (3G)>methyl methacrylate (MMA) and also that for 24 hrs was analogous to that of 3-hr treatment. Changes in fluorescence anisotropy of cells treated with various monomers increased in the order of D-GMA, 1G, TMPT, BG, BD, 3G, MMA. A good correlation was found between these two different methods. These results suggest that the toxicity test using membrane fluidity is a highly useful and excellent method for toxicity testing as an alternative to conventional cytotoxicity testing.
