Abstract
Although dental hard composite resins contain various additives that improve color tones and adhesiveness, the biological safety of the additives have not been extensively investigated. We examined the influence of fluorescent and coloring pigments, which are commom additives to hard composite resin for dental crowns, on colony formation, proliferation, DNA synthesis and protein synthesis of culture cells in addition to cytotoxicity. The coloring pigment inhibited colony formation, proliferation and protein synthesis whereas the fluorescent pigment had no effect on the cultured cells. Further, the low concentration of both pigments significantly enhanced DNA synthesis but did not induce apoptosis or necrosis of the culture cells.
