Abstract
The usefulness of an organ culture method using chick embryo femurs was investigated to evaluate the biocompatibility of biomaterials.Stainless steel wire was inserted into the distal epiphysis of the femur.The tissue reaction around the wire was assessed by histochemical examination.The elements solubled into the tissue around the wire were measured by the X-ray microanalyzer.The femurs did not show growth inhibition.Histologically, however, fibrous connective tissue and a paler cartilage matrix were observed in the tissue adjacent to the wire.In this matrix, the necrotic cells were observed and iron was detected by the X-ray microanalyzer.
