Journal of Japan Endodontic Association
Online ISSN : 2432-4485
Print ISSN : 0389-5238
The Effects of New Development Root Canal Sealers on Human Periodontal Ligment Fibiroblast-like cells
ATSUSHI ONODERAKOUKICHI MATSUMOTO
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1992 Volume 13 Issue 2 Pages 163-171

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Abstract

  A comparison of the effects of newly-developed root canal sealer and commercially available sealers of a cultured human periodontal ligament fibroblastic (HPLF) cells was done in this study. Various root canal sealers were applied to cultured HPLF cells obtained from premolars extracted for orthodontic reasons. Measurement of [H3]-thymidine incorporation and alkaline phosphatase activity was carried out after 24 and 48 hrs.. No significant difference was observed in cellualar DNA synthesis and alkaline phosphatase activity of cells exposed to No.1, No.5, No.6, Canals-N and controls at 24 and 48 hrs.. Canals and Diaket significantly inhibited DNA synthesis and alkaline phosphatase activity at 24 and 48 hrs.. Sealapex had no inhibitory effect on DNA synthesis and alkaline phosphatase activity at 24 hrs., but Sealapex showed inhibitory effect at 48 hrs.. Newly-developed root cadal sealers demonstrated lower toxicity than canals and Diaket in vitro.

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© 1992 Japan Endodontic Association
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