2022 Volume 29 Issue 1 Pages 72-76
The aim of this study was to investigate the effects of culturing human dermal fibroblasts (HDFs) at different temperatures on cell proliferation. In this study, 5×104 cells/dish of HDFs were plated in a 35-mm dish and incubated at 31, 33, 35, 37, and 39°C. After 24, 48, and 72 hours of incubation, the cells were detached and the number of live and dead cells were counted using a hemocytometer. For analysis, the ratio of cell number in each temperature condition divided by the cell number in 37°C incubation at 24 hours was used. The cell viability was also calculated for each condition. For statistical analysis, two-way analysis of variance was used for temperature and time, and one-way analysis of variance was used for cell viability. Bonferroni’s multiple comparison test was performed as post hoc analysis. The results showed significant differences (p＜0.01) for both the main effect and the interaction effect in the two-way ANOVA. The ratio of cells at different incubator temperature settings was higher at both 48 and 72 hours, depending on the higher incubation temperature. No significant difference was observed in cell viability. In conclusion, cell proliferation was lower at 31, 33, and 35°C than at 37°C under the five conditions examined in this study, while cell proliferation was enhanced at 39°C compared to 37°C.
