The Change of Immunoreacive Somatostatin Contents of Cerebrospinal Fluid and Brain in the Development of Cat Amygdaloid Kindling

Abstract

Recently much attention has been paid to neuropeptides as endogenous epileptic inhibitors and promotors. In order to examine the relationship between the development of epilepsy and somatostatin (SRIF), we examined the change of immunoreactive SRIF (IR-SRIF) in cerebrospinal fluid (CSF) and brain at each stage of progression in cat amygdaloid kindling model.
Twenty-five crossbred adult cats were divided into five groups; control group (C), sham operation group (S), stage 4 group (S4) and stage 6 group 2 days (S6-2d) or stage 6 group 2 weeks (S6-2w) after the last stimulation. CSF and 13 individual brain regions were acquired 9+4°C. IR-SRIF was meansured by radioimmunoassay.
IR-SRIF concentrations were significantly higher in the S6-2d group than those of the C group in CSF and several brain regions; left and right amygdala, piriform cortex, and right hippocampus. Those in the S 6-2 w group were also higher in CSF and several brain regions; left and right amygdala, piriform cortex and hippocampus.
Our results suggest that the change in endogenous SRIF may be involved in the development of epilepsy, especially during later stages, in amygdaloid kindled cat and IR-SRIF concentrations in CSF reflect the change of SRIF in the brain.