Unequal Paternal Contribution in an Sugi (Cryptomeria japonica D. Don) Population Made by Mixed Pollen Using Microsatellite Markers

Abstract

We investigated paternal contribution in artificial crossed-populations made by mixed pollen from plus tree clones of Cryptomeria japonica D. Don in Niigata Prefecture. Using six polymorphic microsatellite markers, we identified male parent for each individual tree. We detected significant distortion from equal contribution of male clones in some populations by chi-square test. Therefore, we have to carefully design sugi control pollination with mixed pollens and their use for progeny test.