Abstract
Clone identification was carried out with the combined method of ISSR (Inter Simple Sequence Repeat) analysis (31 markers with 9 primers) and AFLP (Amplified Fragment Length Polymorphism) analysis (26 markers with 2 primer pairs) concerning the superior candidate individuals of Rhus succedanea L. selected by Kyushu Regional Breeding Office, Forest Tree Breeding Center, Forestry and Forest Products Research Institute and Fukuoka Prefectural Government. As the result of the clone identification, 77 individuals are classified into 45 clones, included local cultivars, 9 of Showafuku, 24 of Ikichi and 2 of Oh. In process of identification analysis, although 2 individuals showed identical band pattern but were different from known local cultivars in the ISSR analysis, these 2 individuals showed different fragment patterns in the AFLP analysis were judged as separate genotypes. Cluster analysis and principal component analysis based on the information of the total 204 markers of ISSR, AFLP, and RAPD was also carried out. However there were two genetic groups with low bootstrap probabilities, it was obscureness the relationship between the similarities and geographical distances among selection area of clones. It was considered that artificial transfer of R. succedanea cultivars or mating among cultivated individuals have occurred. The knowledge from this study will help carrying forward R. succedanea breeding program.
