Abstract
In 1927, it was reported by KOKI MASUI that Tricholoma conglobatum (VITT). SACC. (=T. Shimeji KAWAMURA) was a mycorrhizal fungus of Pinus densiflora in synthetic culture, but the mycorrhizal study in nature has not been done.
The writer of this paper has observed mycorrhizal of Quercus serrata near the fruiting bodies of Tricholoma conglobatum in the superficial layer of soil. The fruiting bodies originate very. frequently of a mycelial mass which has been interwoven by the hyphae projected from the surfaces of numerous mycorrhizas.
According to the writer's investigation, Tricholoma conglobatum is a mycorrhiza-former of Quercus serrata in nature. So far as the writer knows, no description about these facts has hitherto been made.
Mycorrhizas of Quercus serrata caused by this fungus are white in colour when fresh, and give off numerous hyphae or hyphal bundles from their surfaces. When the mycorrhizal get old, the mycelium dies and dark rootlets are left.
Microtomic sections of the mycorrhiza stained with FLEMMING's safranin-gentian violet-orange show clearly its internal structure.
The fungous mantle is thick, measuring 21-41μ in thickness.
From the inner part of the mantle, hyphae penetrate inwards between the epidermal cells and from the so-called HARTIG's network. Further inwards, they frequently invade intercellularly the outer layer of the cortex. The host cells contain large quantities of tannic substance.
Fig. 1. Fruiting bodies of Tricholoma conglobatum originated on the mycelial mass produced from mycorrhizas of Quercus serrata. ×1. 2. F, fruiting body; H, humus; R, root.
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Fig. 2. (A) Fruiting bodies of Tricholoma conglobatum attached to the mycorrhizal roots of Quercus serrata. ×1.
(B). Young fruiting bodies of Ticholoma conglobatum occuring on a mycelial mass composed of the mycorrhizas of Quercus serrata. ×1.
F, fruiting body; R, root. _??_
Fig. 3. (A, B). Mycorrhizas of Quercus serrata caused by Tricholoma conglobatum. ×5.2. M, fungous mantle; R, root. _??_
Fig. 4. Mycorrhiza of Quercus serrata caused by Tricholoma conglobatum. ×5.5. O, old mycorrhiza; F, fresh mycorrhiza. _??_
Fie. 5. Mycorrhiza of Quercus serrata caused by Tricholoma conglobatum. ×80. _??_
Fig. 6. Mycorrhiza of Quercus serrata caused by Tricholoma conglobatum. H, humus; M, hyphae; S, sand; _??_
Fig. 7. Young and old mycorrhizas of Quercus serrata caused by Trichotoma conglobatum. H. humus; M, hyphae; O, old mycorrhiza; Y. young mycorrhiza. _??_
Fig. 8. old mycorrhiza of Quercus serrata caused by Trichcloma conglobatum (?) S, small sand. _??_
Fig. 9. Microtomic sections of the mycorrhiza of Quercus serrata caused by Tricholoma conglobatum. (A). Cross section of this mycorrhiza. ×200. (B) Longitudinal section of this mycorrhiza. ×160. C, cortex; 1, intracellular hyphae; M, fungous mantle; Z, central cylinder.
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Fig. 10, Microtomic sections of the mycorrhiza of Quercus serrata caused by Tricholoma conglobatum.
(A). Cross section of this mycorrhiza.
(B). Longitudinal section of an apical portion of this mycorrhiza. H, Hartig's network; 1, intraeellular hyphae. M, fungous mantle; N, nucleus.
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