Abstract
The aim of this study was to clarify the posibilities of a genetic analysis of the isozyme of seedlings at earlier stages. For selfed progenies of twisted-leaf sugi, time serial changes of the peroxidase isozyme patterns of cotyledons and primary leaves were observed from 10 to 12 months after sowing and 9 to 14 months after sowing. In the process, concerning specific bands E or F, seedlings were segregated in a ratio of presence against absence as 3:1. On the back crossed progenies (14 months after sowing), they were segregated in a ratio of 1:1. Thus, it was estimated that each band was due to two alleles (active and inactive) in the same locus, and after the survey of the combined segregation of the bands, the genes for the E and F bands were linked to each other. On the other hand, these two isozyme genes were not linked with a dominant twisted-leaf gene or a recessive dwarf gene which were heterozygous in the twisted-leaf sugi. In conclusion, a genetic analysis of the specific isozyme bands could be made from the patterns of the cotyledons or primary leaves on seedlings about one year old.
