Abstract
We conducted a baiting experiment with stakes of Japanese larch (Larix kaempferi (LAMB.) CARR.) to collect an unidentified basidiomycetous fungus, Basidiomycete-1, which is one of the butt-rot fungi. The stakes were put into the soil to about depths of 20 cm on a general plot (0.98 ha) in a plantation of the Tsukuba University Forests, on five specific plots (100 m2 each) of the Kawakami University Forest, and on a specific plot (0 225 ha) of the Yatsugatake University Forest. One year after, stakes were baited with mycelial strands of the fungus, and 104 strains were obtained by the fungal isolation method. Polyacrylamide gel electrophoresis on vertical slab gels using the hyphae showed that the banding patterns of five enzymes were diploid expressions. We reasoned the genotypes at six loci encoded the five enzymes for the 104 strains, and clarified the allozyme variations and the spatial distributions of genotypes of strains within each plot. Eighteen and seven multilocus-genotypes were observed on the plots of the Kawakami and Yatsugatake Universities Forests, respectively. Concerning the spatial distributions of genotypes of strains on each specific plot, although they were clustered with the same genotypes, the clusters sometimes were mixed with minor different genotypes. This genetic variation may have resulted from hyphal anastomosis because the fruiting bodies of the fungus have not been found.
