Image analysis of C-banding patterns in two herbs: Paris tetraphylla A. Gray and Paris verticillata M. v Bieb. (Liliaceae)

Abstract

In this study, C-banding patterns of the karyotypes of two herbs, Paris tetraphylla and Paris verticillata, were observed and their accurate idiograms were drawn using the CHromosome Image Analyzing System, CHIAS. Then similarities and dissimilarities between the karyotypes of both species were clarified. Our research indicated that the C-bands of the Paris species examined can be classified into four types: 1) Large stable bands such as Cp6, Dp1 and Dq2 of P. tetraphylla and Bp4, Cp6, Dp1 and Ep4 of P. verticillata. 2) Unstable and individual-specific bands. Segments Ap2, Aq2, Bp2, Bq2, Cp2, Cp4, Cq2, Ep2 and Eq2 belong to this type. 3) Very small dot-like bands which appeared only in the segments Dq3 of P. tetraphylla and Dq1 of P. verticillata. 4) C-band positive dots which appeared at the terminal and centromeric regions of all chromosomes of both species.