The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
ARTICLES
Transposable elements in commercially useful insects: I. Southern hybridization study of silkworms and honeybees using Drosophila probes
Kiyoshi KIMURATakashi OKUMURAOsamu NINAKIMargaret G. KIDWELLKoichi SUZUKI
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1993 Volume 68 Issue 1 Pages 63-71

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Abstract
As a first step in surveying transposable elements in silkworms and honeybees, hybridization analyses were carried out using 16 known families of Drosophila transposable elements as probes, jockey and G were the only transposable elements that hybridized with genomic DNA of either honeybees or silkworms under the conditions of this study, jockey hybridized with genomic DNA of both European honeybees (Apis mellifera) and silkworms (Bombyx mori and Antheraea yamamai) and showed significant bands in Southern blots. Banding patterns were highly polymorphic, jockey did not, however, hybridize with any strains of the Asian honeybee (A. cerana). G elements showed a faint signal with the Asian honeybee, but not with any other insects tested. The results suggest that, even though it has some limitations, this approach can be used in practice as a first preliminary step in surveys for the presence of transposable elements in organisms which do not have good genetic information.
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© 1993 by The Genetics Society of Japan
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