The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
ARTICLES
DNA fingerprinting of animal genomes by CA-repeat primed polymerase chain reaction
Yasuyuki ISHIBASHISyuiti ABEMichihiro C. YOSHIDA
Author information
JOURNAL FREE ACCESS

1995 Volume 70 Issue 1 Pages 75-78

Details
Abstract
CA-repeat primed polymerase chain reaction (CAP-PCR), using degenerate primers which anneal at the ends of (CA)n sequences in eukaryotic gejiomes, was attempted to assess its potential to monitor the genomic polymorphisms in various animals. Three mammalian, three avian, one fish and one insect species were examined and all showed primer-specific DNA fingerprints by CAP-PCR. Polymorphic bands observed in a laboratory-bred vole family were segregated in Mendelian manner. The present CAP-PCR DNA fingerprinting therefore is a simple and useful method for examining genomic variations in most animals without prerequisite knowledge of DNA sequences.
Content from these authors
© 1995 by The Genetics Society of Japan
Previous article Next article
feedback
Top