Abstract
By the fluorescence microscopy, the auther studied on the relation between the phenomenon of the appearance of autofluorescent granules in living larvae of Ascaris eggs and the maturity of the larvae.
Results obtained as follows:
In the 8-9 days larvae after the formation of embryos, fine granules (0.5-0.9μ in diameter), in which faint yellow autoflurescence was observed, were found chiefly in contact with the intestinal cells. Such larvae increased during 10-13 days after the embryo-formation, and the fluorescence color changed to golden yellow. These autofluorescent fine granules were scattered or standing in lines along the intestinal cells.
On the other hand, by the infection test with mice, it was confirmed that the infective larvae remarkably increased during 8-13 days after the embryo-formation. From the above observations, it was considered that the appearance of yellow autofluorescent fine granules showed the maturity of the larvae.
The infective power got to the peak during 16-30 days after the embryo-formation, and it fell down gradually after that, and the autofluorescence of the granules changed to golden yellow accompanied with transformation to round masses.
