Abstract
Histochemical studies were carried out on lipid, lipoid, glycogen, nucleic acid, phosphatase, calcium and waste pigment, in order to clarify the chemical property of the autofluorescent fine granules in the infective larvae of Ascaris.
Further more the author studied on the localization of these substances in the larva body.
The results obtained as follows:
1) The autofluorescent granules may be lipogenic pigment (waste pigment), which is approximate to ceroid.
In the intestine cells the same pigment was found. And the increase of autofluorescence of the granules with age of larvae may be caused by the increase of lipogenic pigment and calcium.
2) Lipid, that is, fatty acid and soap (perhaps, oleinic acid and calcium salt), cholesterine ester, phosphatide, glycogen, RNA and acid phosphatase were positive in the autofluorescent granules.
3) Lipid, namely, neutral fat, fatty acid and calcium salt, cholesterine ester, phosphatide, glycogen and DNA were positive in the intestine cells.
4) Lipid and glycogen contained in the autofluorescent granules and intestine cells decreased remarkably after one year. From this result, it is considered that the infective larva in egg-shell consumes lipid and glycogen contained in the autofluorescent granules and intestine cells as nutriment.
