Abstract
Studies on alkaline and acid phosphatase activities in male rabbit brains were carried out under different physical conditions. The environmental conditions used were similar in the previous report. After the animals were killed by air puncture, the brain was removed and separated rapidly into 7 parts; telencephalon, diencephalon, the upper and lower part of mesencephalon, cerebellum, pons and medulla oblongata. The each part of brain was ground in ice-cold water solution by glass-homogenizer.
The phosphatase activity was determined by KING and ARNSTRONG's method, modified by SHIBATA. The following results were obtained.
Alkaline phosphatase activity was increased significantly in each part of rabbit brain under the noisy, cold and immobilized condition in comparison with controls. The increasing value under the cold and immobilized condition was higher than that of noisy condition.
Acid phosphatase activity was increased significantly in each part of brain under the cold condition, in the part of brain except pons and medulla oblongata under the immobilized condition and in telencephalon and medulla oblongata under the noisy condition.
