Abstract
Effects of sulfite on glucose and amino acid metabolism were examined with the following results:
1. The inhibitory effect on glucose oxidation of intact cells was not clearly observed with 1mM sulfite in the reaction medium, but TCA cycle metabolites such as malate, pyruvate and succinate showed a fairly good inhibition with the same concentration of sulfite. Sulfite showed a greater inhibition to glutamate and alanine oxidation of intact cells of Staphylococcus aureus than that of Escherichia coli and Proteus vurgaris.
2. By pre-incubation with sulfite, lactic, succinic dehydrogenase and L-amino acid oxidase in cell free extracts showed considerable low activity.
Increased concentration of sulfite and prolongation of pre-incubation time resulted in enlargement of inhibitory activity. Aminotransferase of Escherichia coli was also inhibited with sulfite.
