Abstract
Biochemical effects in mice and cytotoxicity in a cultured cell line of a poisonous mushroom extract, Amanita abrupta were studied. The aqueous extract of the mushroom was injected intraperitoneally into male SPF ddy mice and the changes of components and enzymes in blood, liver and urine were determined. The cytotoxicity of the mushroom extract was also studied in terms of the effect on the colony formation of KB cells.
Biochemical changes were observed after a latent period of 6h after the injection; blood glucose and liver glycogen decreased to 60% and 10% of the control levels, respectively. Serum glutamate-oxalacetate transaminase (GOT) and blood urea nitrogen (BUN) increased to 3-fold that of the control levels at 12-15h after the injection and remained at these high levels for 24h.
Urinary parameters were significantly affected by the mushroom extract.
The mushroom extract showed a marked inhibitory effect on the plating efficiency of KB cells.
