Abstract
Adoptive immunotherapy was applied to 2 maxillary cancer cases and 1 lingual cancer case (squamous cell carcinoma). Peripheral blood lymphocytes obtained through leukapheresis were cultured for 3 days with allogeneic tumor cell line originated maxillary squamous cell carcinoma, and further incubated in recombinant interleukin-2 (rIL-2) to generate allogeneic tumor and lymphokine activated killer (Allo-TLAK) cells. AlloTLAK cells were infused directly into the maxillary artery for the maxillary cancer cases and into the lingual artery for the lingual cancer case. Tumor staged as T3 of one maxillary cancer case completely disappeared after infusion of 4.5×109 AlloTLAK cells. Objective tumor regression was observed in the other maxillary cancer case after infusion of 2.74×109 AlloTLAK and 0.76×109 ATLAK cells. After completion of adoptive transfer of these cells, total maxillectomy was performed. Tumor size of lingual cancer was markedly reduced from T3 to T1 in clinical estimation after infusion of 2.9×109 AlloTLAK cells. This case underwent surgery to remove 1/3 of the tongue along with radical neck dissection. Surgically resected tissues of maxillary cancer and lingual cancer were histologically examined. They revealed tumor degenetration with infiltration of lymphocytes, scavenger macrophages. Side effects were minor. Such as, a slight fever and blood eosinophilia.
