Direct Observation of Fish Spleen by an Abdominal Window Method and Its Application to Exercised and Hypoxic Yellowtail

Abstract

The spleen of the yellowtail (Seriola quinqueradiata) was directly observed through a window opened at the abdomen during exercise experiments by continuous chasing and during hypoxia experiments by nitrogen bubbling.The spleen decreased above 30% in length, 60 % or more in weight and 70% or more in hemoglobin content, either by exercise or by hypoxia.